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ISOLATION AND MOLECULAR IDENTIFICATION OF TWO STRAINS OF MICROALGAE FROM SIDI AMEUR SALT LAKE IN ALGERIA

Algerian Journal of Arid Environment “AJAE”

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ISSN 2588-1949
2170-1318
 
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Title Statement ISOLATION AND MOLECULAR IDENTIFICATION OF TWO STRAINS OF MICROALGAE FROM SIDI AMEUR SALT LAKE IN ALGERIA
 
Added Entry - Uncontrolled Name ACHOUR Hafsa, YAICHE
Amel, DOUMANDJI
Noureddine, BOURAS
Nasserdine, SABAOU
Patricia, ASSUNҪÃO
)Laboratoire de Recherche de Biotechnologie des Productions Végétales, Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie Université de Blida 1, 09000, Blida, Algérie
)Laboratoire de Recherche de Biotechnologie des Productions Végétales Département Agroalimentaire, Faculté des Sciences de la Nature et de la Vie Université de Blida 1, 09000, Blida, Algérie
Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, 47000, Ghardaïa, Algérie (4) Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algérie
) Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algérie
) Instituto Tecnológico de Canarias (ITC), Departamento de Biotecnología, División de Investigación y DesarrolloTecnológico, Pozo Izquierdo, 35119, Sta. Lucía, Canary Islands, Spain
 
Summary, etc. Microalgae constitute an excellent feedstock of valuable compounds which have varied applications in the nutraceutical and pharmaceutical industries. In this study, the isolation of two microalgal strains, DunaDZ2 and DunaDZ3, from Sidi Ameur salt lake was done by streaking water on solid f/2 medium. The strains were subjected to morphological identification as well as molecular identification by sequencing genes ITS and rbcL. The strain DunaDZ2 presented a palmella stage (colonial aggregates of cells) throughout all of its life cycle (length 5.5 ± 1.8 µm and width 4.8 ± 1.2 µm). By contrast, DunaDZ3 presented ellipsoid shape of about 14.6 ± 1.7 µm length and 6.9 ± 1.3 µm width. Phylogenetic analysis based on ITS and rbcL genes revealed that both strains are members of the genus Dunaliella, and were grouped in the same clade with different strains of Dunaliella viridis (96.9 to 98.7% of similarity for ITS gene, and 96.4 to 98.1% for rbcL gene). The species Dunaliella viridis is considered as promising feedstock for applications in the production of biofuels and as a nutritive food source for aquatic organisms.
 
Publication, Distribution, Etc. Université Kasdi Merbah Ouargla
 
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https://journals.univ-ouargla.dz/index.php/AJAE/article/view/212
 
Data Source Entry Algerian Journal of Arid Environment “AJAE”; Vol 8 No 1 (2018): Vol. 8, n° 1, juin 2018
 
Language Note eng
 
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