IN VITRO ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS OF ALGERIAN ALLIUMpdf PLANT (Allium triquetrum L.)

A MENACER; M N BOUKHATEM; A BENHELAL; F SAÏDI

A MENACER Laboratoire Biotechnologies, Environnement et Santé, Département de Biologie et Physiologie Cellulaire, Faculté des Sciences de la Nature et de la Vie, Université –Saad Dahleb- Blida 1, Blida, Algeria.
M N BOUKHATEM Département de Biologie et Physiologie Cellulaire, Faculté des Sciences de la Nature et de la Vie, Université –Saad Dahleb- Blida 1, Blida, Algeria.
A BENHELAL Laboratoire d’Analyses Médicales, Ouled yaich, Blida, Algeria.
F SAÏDI Laboratoire Biotechnologies, Environnement et Santé, Département de Biologie et Physiologie Cellulaire, Faculté des Sciences de la Nature et de la Vie, Université –Saad Dahleb- Blida 1, Blida, Algeria.

Keywords: Antioxidant activity, Allium triquetrum L, extracts, polyphenols, flavonoids

Abstract

To enrich the knowledge of new bioactive molecules and their natural resources, the present study aims to value an endemic plant growing in Algeria, named Allium triquetrum L., by evaluating the antioxidant activity of aqueous and methanolic extracts from the leaves (AEL, MEL) and the bulbs (AEB, MEB), byusing DPPH scavenging and FRAP methods, with a search of main chemical groups and determination of total phenolic compounds. Phytochemical study revealed mainly, the presence of flavonoids, saponins and glycosides. Furthermore, AEL has presented the greatest levels of polyphenols (14.31 ± 0.36 mg GAE/g dw) and flavonoids (22.12 ± 0.33 mg QE/g dw). The aqueous extracts have a high antioxidant capacity comparing to the methanolic extracts (EC50: AEL = 0.97 mg/ml, AEB = 1.36 mg/ml). Our results indicate clearly that Allium triquetrum extracts show important antioxidant activity and could be used as a new source of natural antioxidants molecules