INSECTICIDAL ACTIVITY OF ETHYL ACETATE EXTRACT OF Borago officinalis L. (BORAGINACEAE) AGAINST Ctenocephalides felis And Archaeopsylla erinacei (SIPHONOPTERA, PULLICIDAE)

  • Djillali EL HADDAD Laboratory of Valorization and Conservation of Biological Resources, Faculty of Sciences, University M’Hamed Bougara of Boumerdes, 35 000 Boumerdes, Algeria
  • Souheyla TOUBAL Laboratory of Valorization and Conservation of Biological Resources, Faculty of Sciences, University M’Hamed Bougara of Boumerdes, 35 000 Boumerdes, Algeria.
  • Ouahida BOUCHENAK Laboratory of Bioinformatics, Applied Microbiology and Biomolecules, Faculty of Sciences, University M’Hamed Bougara of Boumerdes, 35 000 Boumerdes, Algeria.
  • Karima YAHIAOUI Food Technology Research Laboratory, Faculty of Technology, University M’Hamed Bougara of Boumerdes, 35 000 Boumerdes, Algeria.
  • Maroua MERAH Laboratory of Biotoxicology, Pharmacognosy and Biological valorization of Plants. University Dr. MoulayTahar, PO Box 138 Al-Nasr District 20000, Saida-Algeria.
  • Karim ARAB Laboratory of Valorization and Conservation of Biological Resources, Faculty of Sciences, University M’Hamed Bougara Boumerdes, 35 000 Boumerdes, Algeria.
  • Idir BITAM Laboratory Biodiversity and Environment: Interactions, Genomes, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, 16 000 Algiers, Algeria, Higher School of Food Sciences and Agroalimentary Industries 16 000 Algiers, Algeria
Keywords: Insecticidal activity, Fleas, HPTLC, Borage, Vector-borne diseases

Abstract

Vector-borne diseases account for more than 17% of infectious diseases, and cause more than one million deaths each year. However, the misuse of chemical insecticides has resulted in insect resistance. This work concerns the evaluation of the insecticidal activity of the ethyl acetate extract of Borage (Borago officinalis L.), a plant widely distributed in northern Algeria, on the cat flea Ctenocephalides felis and the flea of the Hedgehog Archaeopsylla erinacei, carriers of serious vector diseases. For that, adult flea specimens of C. felis and A. erinacei were exposed to a series of concentrations of plant extracts. (50 mg / ml, 25 mg / ml, 12.5 mg / ml and 6.25 mg / ml) using pre-impregnated wattman paper strips. The plant extract was obtained by fractionation using appropriate solvents; it was then characterized by high performance thin layer chromatography (HPTLC). The extraction yield was 4.87% with a flavonoid concentration of 120.99 ± 0.81 μgeq Q / g extract. Chromatographic characterization by HTPLC reveals the presence of rutin, rhamnetin and cyanidin-3-glucoside in this extract. The results obtained show that the ethyl acetate extract of Borage greatly reduces the population of the two species of flea A. erinacei and C. felis after 48 hours of contact. The LC50 values were 9.74 mg / ml and 11.24 mg / ml respectively. The mortality kinetics results show TL50 values of 7.29h and 8.51h on the same species respectively.

Published
2021-01-04