BIOETHANOL ANDSINGLE-CELL PROTEINPRODUCTION BYErwiniachrysanthemiEC3665FROM WASTEDISTILLERYPLANT

Abstract

The distillation of essential oils from aromatic and medicinal plants causes formation of lignocellulosic waste (cellulose, hemicellulose, and phenolic compounds) in considerable quantities (32g example of waste per 100g rosemary distilled). The objective of this work is to deal, with bioprocessing, lignocellulosic waste from the distillation of rosemary to produce bioethanol. This waste was submitted to steam for the depolymerization. Then they were treated with sulfuric acid to liberate simple sugars. After settling and filtration, the filtrate was treated with calcium hydroxide (CaOH2) to precipitate the phenolic compounds which were reduced by 60%. The filtrate is supplemented with yeast extract, ammonium sulfate ((NH4) 2SO4) and disodium phosphate (Na2HPO4) to serve as fermentation medium for bacterial strains Erwinia chrysanthemiand Klebsiella oxytocaEC3665 for producing bioethanol. Bacterial fermentation of waste were served for the production of proteins of unicellular organisms.The results show that treatment of lignocellulosic material release fermentable sugars (Glucose 9.6 g.l-1; Xylose 7.33 g.l-1; Mannose2.37g.l-1; Galactose 1.2 g.l-1). These sugars have produced bioethanol with a higher yield with Erwinia chrysanthemiEC3665 (0.25 gP /gS) against Klebsiella oxytoca(0.16 gP /gS).This work has helped to develop biotechnological processes feasible at all solid waste from the pharmaceutical industry and/or agrifood for bioethanol production and consequently contribute to sustainable development.