ANTIMICROBIAL SCREENING OF Saccharothrix tamanrassetensis DSM 45947 AND EFFECT OF CARBON AND NITROGEN SOURCES ON ANTIBIOTIC PRODUCTION

Leila BAKOUR KERAMANE; Hadjira BOUDJELLA; Atika MEKLAT; Noureddine BOURAS; Nasserdine SABAOU; Florence MATHIEU

Leila BAKOUR KERAMANE Laboratoire de Biologie des Systèmes Microbiens (LBSM) Ecole Normale Supérieure de Kouba, Alger, Algeria
Hadjira BOUDJELLA )Laboratoire de Biologie des Systèmes Microbiens (LBSM) Ecole Normale Supérieure de Kouba, Alger, Algeria
Atika MEKLAT Département de Biologie et Physiologie Cellulaire, Faculté des Sciences de la Nature et de la Vie, Université Saâd Dahleb, Blida 1, Algeria
Noureddine BOURAS Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre Université de Ghardaïa, Ghardaïa, Algeria
Nasserdine SABAOU Laboratoire de Biologie des Systèmes Microbiens (LBSM) Ecole Normale Supérieure de Kouba, Alger, Algeria
Florence MATHIEU Laboratoire de Génie Chimique, Université de Toulouse, CNRS, Toulouse, France

Keywords: Saccharothrix, tamanrassetensis, antagonist activity, PCR, genes, carbon and nitrogen sources, production kinetics, HPLC

Abstract

The Algerian Saharan actinobacterium Saccharothrix tamanrassetensis DSM 45947, exhibited an antagonist activity against Gram-positive bacteria, yeasts and filamentous pathogenic, toxigenic and phytopathogenic fungi. The antimicrobial potential of the strain was examined by PCR for the presence of type I and II of polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) gene clusters. The latter gene was detected, but the first two genes were absent. Production of antimicrobial (antifungal and antibacterial) activity was investigated by following kinetics in a semi-synthetic broth supplemented with various carbon and nitrogen sources. The highest activity was obtained with corn steep liquor and sucrose. Antimicrobial compounds were extracted from the culture filtrate. A strong activity was detected in the aqueous phase extract; however the activity in the organic extract was weak. The crude extracts were injected in HPLC for purification. Each fraction was tested for antimicrobial activity. The results revealed 7 active peaks in the aqueous extract, suggesting the presence of novel antibiotics.