EFFET HEPATO-PROTECTEUR D’EXTRAITS BRUTS DE POLYSACCHARIDES DE FEUILLES D’Oudneya africana R. Br. (BRASSICACEAE) SUR L'INFLAMMATION AIGUË INDUITE PAR L'ACETATE DE PLOMB CHEZ DES RATS Wistar albino

Abstract

.- This work focuses on the study of polysaccharides from the leaves of Oudneya africana R. Br. (Brassicaceae), a spontaneous plant with a medicinal character harvested in the region of M'Rara wilaya of El Oued (northern Algerian Sahara). The extraction of water-soluble polysaccharides (PHOA) gave a mass yield of 7.24%. The determination of the composition of PHOA obtained, by colorimetric assays, indicates levels of 50.63±0.07% in total sugars, 47.95±0.002% in neutral sugars and 21.20±0.001% in proteins. Analysis of the monosaccharide composition by high performance anion exchange chromatography coupled with a pulsed amperometric detector (HPAEC-PAD) found the presence of l-arabinose (11.4%), d-galactose (13.3%), d-glucose (19.7%), d-mannose (46%), d-galacturonic acid (8.3%), and d-glucuronic acid (1%). The study of the hepatoprotective activity of the extract is carried out in vivo on male and female rats of the Wistar albino strain by oral poisoning with lead acetate. At a concentration of 100mg/kg, the PHOA fraction shows antioxidant activity by significant suppression of the increase in malondialdehyde (MDA) levels and the decrease in reduced glutathione (GSH) levels in liver and kidney tissues observed after exposure to lead. Histopathological analysis of the livers indicates that PHOA has a hepatoprotective effect in female rats while they note a decrease in the intensity of hepatotoxicity in males, which makes them a potential agent for the prevention of liver damage.