VALORISATION OF DATE WASTE FOR THE PRODUCTION  OF PROBIOTIC BACTERIAL BIOMASS

SIFOUR SIFOUR Mohamed; OULED-HADDAR Houria; AISSAOUI Salima; IDOUI Tayeb; ZAHRIOU Rokia; ZIDANE Sara

SIFOUR SIFOUR Mohamed Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences University Mohamed Seddik Benyahia of Jijel, Algeria
OULED-HADDAR Houria Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences University Mohamed Seddik Benyahia of Jijel, Algeria
AISSAOUI Salima Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences University Mohamed Seddik Benyahia of Jijel, Algeria
IDOUI Tayeb Laboratory of Biotechnology, Environment and Health, Faculty of Nature and Life Sciences, University Mohamed Seddik Benyahia of Jijel,, Algeria
ZAHRIOU Rokia Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences University Mohamed Seddik Benyahia of Jijel, Algeria
ZIDANE Sara Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences University Mohamed Seddik Benyahia of Jijel, Algeria

Keywords: Biomass production, date juice, Lactobacillus plantarum, Plackett-Burman design, probiotic

Abstract

This study aimed to valorise date waste by improving biomass production of Lactobacillus plantarum G₁ using date juice as carbon source. The physicochemical analysis indicated that the date juice contained a high amount of sugars easily assimilated which make it favorable for the growth of the bacterial strain. The results showed that the date juice with 12% of total sugar supplemented with salts and organic nitrogen found in MRS medium produced a higher biomass yield when compared to biomass production in MRS medium and in date juice alone. Plackett-Burman statistical experimental design was used to screen the most important nutrients influencing lactic acid biomass production. Seven variables including date extract were screened for their effect on Lactobacillus plantarum biomass production. Results from this analysis demonstrated that the date extract, yeast extract and peptone were the most significant factors.